Igenex testing needed & WB Breakdown
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Author:  Cog1st [ Mon Feb 18, 2008 11:58 am ]
Post subject:  Igenex testing needed & WB Breakdown

Igenex testing needed & WB Breakdown

NOTE: Do not rely soley on testing for Lyme Disease. It is important to know that Lyme testing is very unreliable and will more times than not produce a "false-negative" blood test result. It is a clinical diagnosis, this is stated clearly by the CDC. Meaning that when symptoms of Lyme disease are present, despite a negative test, it should be treated by a qualified Lyme literate Md. (see Center for Disease Control, "clincial diagnosis" here: ... nical.html )

ImageWestern Blot Test

When testing through Igenex it is imperative to be tested for co-infections as well as Lyme. The following blood tests should be run:

Lyme Western Blot IgG & IgM (check both test #'s 188,189)

Babesiosis - B.microti IgG & IgM Antibody (test #200)

B.duncani IgG & IgM (WA-1 - West coast testing) (test #720)

Ehrlichia Chafeensis (HME) (test #203)

Anaplasma Phagocytophila (HGE) (test #206)

Bartonella - B. henselae IgG & Igm Antibiody (test #285)


The following is a breakdown of the Western blot only. As co-infections will show their own titers. A positive IgM is a sign of a current/active infection. IgG can be a sign of an older/longer standing infection and if symptomatic treatment is needed. If the tests are negative, remember no LD test is 100% reliable and sometimes a body is too weak to "mount" an accurate positive result. LD is a clinical diagnosis and ONE band in the test being positive can indicate Lyme Disease is present. Please keep reading.


IGeneX also now offers a new 30-31kDa Confirmation IgG and IgM test. If results from the initial Western Blot are positive for bands 30 or 31, it is possible that these could be due to cross-reactivity with several different types of viruses. In this confirmatory test, highly specific recombinant antigens are used to validate that the positive result is not due to cross-reaction with viruses.
If ONLY band 31 is positive and no other specific bands are positive, it is recommended to have tests #488 and #489 31 kDa epitope Igm/Igg to make sure borrelia hasn't crossed reacted with several possible viruses....but AGAIN ONLY IF BAND 31 ALONE is positive.

There are nine known Borrelia burgdorferi genus specie specific KDA Western Blot antibodies (bands): 18 23 30 31 34 37 39 83 and 93. Only one of these Borrelia burgdorferi genus specie specific bands is needed to confirm that there is serological evidence of exposure to the Borrelia burgdorferi spirochete and can confirm a clinical diagnosis of Lyme disease. (More info pages 12 & 13 "Western Blot Made Easy":

Igenex Western Blot Break Down by band
9 cross-reactive for Borrellia
12 specific for Bb
18 highly specific to Lyme (Many LLMD's say if this band alone is positive, you have lyme - see link above)
20 cross-reactive for Borrellia
21 unknown
22 specific for Bb, probably really the 23/25 band
23-25 outer surface protein C (OspC), specific for Bb
28 unknown
30 unknown; probably an outer surface protein; common in European and
one California strain - Has cross-reactivity with several different types of viruses
31 outer surface protein A (OspA), specific for Bb - Has cross-reactivity with several different types of viruses
34 outer surface protein B (OspB); specific for Bb
35 specific for Bb
37 specific for Bb
38 cross-reactive for Bb
39 is a major protein of Bb flagellin; specific for Bb
41 flagellin protein of all spirochetes; this is usually the first to appear after a spirochete infection but is NOT specific to Lyme (i.e, other spirochete diseases have flagellas - see link above "Western Blot Made Easy" for more info)
45 cross-reactive for all Borellia
50 cross-reactive for all Borrellia
55 cross-reactive for all Borrellia
57 cross-reactive for all Borrellia
58 unknown but may be a heat-shock Bb protein
60 cross reactive for all Borrellia
66 cross-reactive for all Borrelia, common in all bacteria
83 specific antigen for the Lyme bacterium, probably a cytoplasmic membrane
93 unknown, probably the same protein in band 83, just migrates differently in some patients[/b]

An IgM positive test result means more recent & likely currently active infection. IgG positive means previous exposure to the bacteria or older infection of Lyme. If no symptoms are present with IgG positive, it may mean an "inactive" LD infection. No matter the results, please remember LD is a clinical diagnosis, you can have a negative test and still have Lyme Disease and the co-infections, even through the best labs. If you have symptoms, it is imperative to see a Lyme literate Md so that you can be properly diagnosed and treated. If your test reads "CDC negative please read the following link (both pages) at it explains how this occurs.

Author:  Cog1st [ Mon Feb 18, 2008 3:36 pm ]
Post subject:  Re: Igenex testing needed & WB Breakdown


Organism Prevalence
Babesia 8-20% *
Bartonella 40-70%
Ehrlichia 10-50%
Borrelia 18-40%
Mycoplasma 25-70%
* Some Lyme doctors have
estimated that the presence of
Babesia infection may actually be
70% or higher based on their clinical
When reviewing the symptoms
caused by the various coinfections,
it becomes clear that
there is significant overlap
between the symptoms which
result from co-infections and those
that are caused by Borrelia itself.
Though Borrelia may be the ringleader,
it is critical that one not
overlook the very real and almost
certain reality that co-infections
may be a significant part of one's
current condition.
In summary:
• Co-infections are the RULE, not
an exception.
• The average child with Lyme disease
has 2-5 co-infections with an
average of 3.
• Treatment of co-infections is
required and often, they must be
treated before or concurrent with
the Borrelia treatment itself.
• If you don't test for and treat coinfections,
you are not putting
yourself in a good position for
• Most people with chronic Lyme
have 1 or more co-infections.
• Co-infections require different
treatments in many cases. Do not
assume that you are covering them
with only the Lyme treatment.
• Co-infection testing is often
unreliable as well and you need to
repeat them over time.

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